Abstract: In order to realize wireless acquisition and transmission of the sensing signals of the smart cement-based sensors for stress/strain monitoring of concrete structures, the conception and the sensing principle of smart cement-based sensors were introduced, and the design method was presented based on the characters of the signals of smart cement-based sensors and the modularized program. The operating principle of the wireless acquisition and transmission system was especially analyzed, and the hardware circuit and the software program chart were presented, then every hardware element and the integration method were discussed in detail. The results showed that the presented wireless acquisition and transmission system of the sensing signals of smart cement-based sensors had such advantages as simple circuit easy to realize, high reliability, low cost, convenient setting and good practicability, and it was suitable for application in health monitoring of concrete structures.