Abstract: According to the inherent characteristics of ultrasonic signals, the paper proposes a high-speed ultrasonic signal acquisition system. The high frequency signal coming from ultrasonic sensor is transferred to the PC through PCI Bus after being high frequency amplified, A/D converted and high speed accumulated. It solves the problem of high-speed, big capacity storing and transmitting of the spot testing data. The proposed system can be applied in various ultrasonic signal acquisition and processing because of its high flexibility and portability.