Abstract: The acoustic impedance of each layer in the multilayer media structure varies greatly. The interlayer materials are often have characteristics of high acoustic attenuation and frequency dispersion. The general ultrasound scanning images are not sufficient to determine the structural information between layers. In this paper, the propagation characteristics of high frequency ultrasonic waves entering the resin medium simulating human fingerprint after passing through the water and glass layers are analyzed. The high frequency ultrasonic transducer is used to stimulate and collect the ultrasonic signals with wide spectrum in the multilayer medium by C-scan to gain the time domain waveform data. By virtue of the waveform characteristics obtained by the propagation theory, the characteristic information of the interface under the glass layer is extracted by using a specific time domain peak imaging algorithm, thereby obtaining a high precision ultrasonic image for fingerprint under glass layer.